Analysis Note

ControlPOSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) rat fibrosarcoma cells or rat keratinocyte CCL-4.

Application

Anti-MMP-13 Antibody, clone LIPCO IID1 is an antibody against MMP-13 for use in WB.

Research CategoryCell Structure

Western Blotting (Lyons, 1991; Lyons, 1993): 1:200-1:400 at room temperature for 2 hours.

Immunofluorescence

Optimal working dilutions must be determined by end user.

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rat MMP-13 from BC-1 cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Specificity

It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Antibody shows no cross-reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster.

Cellular Localization:

Cytoplasmic

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.